The role of the phosphoenolpyruvate phosphotransferase system in the transport of N-acetyl-D-glucosamine by Escherichia coli.
نویسنده
چکیده
The properties of an N-acetyl-d-glucosamine-transport system have been studied by following the intracellular accumulation of methyl 2-acetamido-2-deoxy-alpha-d-[1-(14)C]glucoside by Escherichia coli. The same analogue was used to assay phosphoenolpyruvate phosphotransferase activity of toluene-treated cells. Transport and phosphorylation are induced by growth on d-glucosamine or N-acetyl-d-glucosamine. Mutants resistant to N-iodoacetyl-d-glucosamine are defective in the uptake and phosphorylation of the labelled glycoside.
منابع مشابه
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 118 1 شماره
صفحات -
تاریخ انتشار 1970